Signalling

Part:BBa_K3425108

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-23)


pCadBA testing construct (pSB1K01)

BBa K3425108 is a pCadBA testing construct containing the pCadBA operator and promoter BBa_K3425101 followed downstream by the RBS BBa_B0034 and mRFP1 (BBa_E1010) expressed in pSB1K01.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2179
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2179
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2179
    Illegal XhoI site found at 1029
    Illegal XhoI site found at 2055
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2179
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2179
    Illegal PstI site found at 12
    Illegal AgeI site found at 2912
    Illegal AgeI site found at 3024
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2185
    Illegal SapI.rc site found at 5


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Categories
Parameters
None