Signalling
Part:BBa_K3425108
Designed by: Rubén Crespo Blanco Group: iGEM20_UofUppsala (2020-10-23)
pCadBA testing construct (pSB1K01)
BBa K3425108 is a pCadBA testing construct containing the pCadBA operator and promoter BBa_K3425101 followed downstream by the RBS BBa_B0034 and mRFP1 (BBa_E1010) expressed in pSB1K01.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2179
Illegal PstI site found at 12 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2179
Illegal PstI site found at 12 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2179
Illegal XhoI site found at 1029
Illegal XhoI site found at 2055 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2179
Illegal PstI site found at 12 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2179
Illegal PstI site found at 12
Illegal AgeI site found at 2912
Illegal AgeI site found at 3024 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2185
Illegal SapI.rc site found at 5
[edit]
Categories
Parameters
None |